Activation of innate immunity is regulated by a variety of signaling molecules within

the immune cells. The present thesis was aimed to improve our understanding

innate signaling mechanism and their possible use as bio-indicators of exposure and

disease. The first part of the thesis deals with the involvement of TOM1L1 (Target

of Myb1 like 1) in innate immune signaling and regulation of inflammatory cytokines

in immune cells (study I and II). The initial event of T-cells activation depend

on the recruitment of Src family kinases Fyn and Lck, leading to interleukine-2 (IL-

2) production in T cells. Understanding the regulatory aspects of IL-2 induction in

T-cells is of importance as IL-2 is a key regulator for T-cell proliferation and survival.

Interaction screening indicated the ability of TOM1L1 protein to interact with

Fyn, and Lck, that is important for IL-2 production in Jurkat T-cells. TOM1L1

silencing decreased the levels of CD3/CD28 dependent induction of IL-2 in Jurkat

T-cells, and LPS dependent induction of TNF-α in THP-1. Furthermore, overexpression

of TOM1L1 in Jurkat T-cells causes an increase of STAT3 expression. This

was accompanied by an increase in the levels of IL-1β dependent induction of IL-6

and TNF-α in THP-1 cells. These results indicate that TOM1L11 participate in

regulation of innate immune response. The second part of the thesis deals with

development of innate immune signaling responses used as a diagnosis tools for

disease and exposure (study III and IV). Inflammatory diseases are associated with

innate immune reactions. In response to inflammation, the immune cells release

inflammatory cytokines such as IL-1-β, IL-2, IL-6, IL-10, TNF-α and CXCL8. These

cytokines are regulated by stress related kinases include MAP kinase proteins such

as ERK1-2, JNK, and MAPK p38, through activation of transcription factors AP-1,

ATF-2, and NF-AT. In a clinical study, it was observed that activated MAPK p38

has a potential role in the regulation of IL-10 expression in intermittent claudication.

However, expression of IL-10 and MAPK p38 was opposed in stable angina

group. Therefore, targeting MAPK p38 in inflammatory disease such as cardiovascular

diseases, diabetes, and rheumatoid arthritis might be useful in development of

treatment strategies. Innate immune reactions can also be used to monitor stress

related inflammatory responses following environmental exposure of immune cells.

Inflammatory responses of exposure were studied by in vitro exposure to waters

from sewage treatment works and recipient waters. The analysis shows that exposure

to inland waters can result in activated immune responses and that these responses

are both site dependent and vary over time.

Keywords: Innate immunity, TOM1L1, inflammatory responses.

The present paper involves a detailed evaluation of specific steroid biomarkers by gas chromatography– mass spectrometry (GC–MS) and GC-metastable reaction monitoring (MRM) analyses of several crude oils and source rocks from the East Sirte Basin. 24-Norcholestanes, dinosteranes, 4a-methyl-24-ethylcholestanes and triaromatic steroids have been identified in both source-rocks and crude oils of the East Sirte Basin. Diatoms, dinoflagellates (including those potentially associated with corals) and/or their direct ancestors are amongst the proposed sources of these biomarkers. These biomarker parameters have been used to establish a Mesozoic oil–source correlation of the East Sirte Basin. Hydropyrolysis of an extant coral extract revealed a similar distribution (although immature) of dinosteranes and 4a-methyl-24-ethylcholestanes also observed in the Sirte oils and source-rocks. This is consistent with the presence of dinoflagellates present during the deposition of the Mesozoic aged East Sirte Basin Formations. A good data correlation for the rock extracts revealed a similar distribution of 3,24-dimethyl triaromatic steroids, 3-methyl-24-ethylcholestanes, 4-methyl-24-ethylcholestanes and 2-methyl-24-ethylcholestanes observed in one of the oil families and associated source-rocks for the East Sirte Basin.

 Abstract: The paper presents an adaptive intelligent control method to overcome effects of some indeterminate and undealt factors that a DC .drive is suffered. In the speed loop, we use a three-layer neural networks through a backpropagation (BP) algorithm out of line learning to realize the fuzzy-control tactics. We use unit neuron through Hebb algorithm on-line dynamic learning to realize adaptive mechanism. The simulation is based on a MATLAB6.0 neural networks toolbox with simulink. The results of the simulation show that adaptive intelligent control method enables the system to have good dynamic and stability performance. The proposed method develops the use of simulink in the field of electrical drive of adaptive intelligent control.

Biomarker ratios, together with stable carbon (d13C) and hydrogen (dD) isotopic compositions of individual hydrocarbons have been determined in a suite of crude oils (n = 24) from the East Sirte Basin to delineate their sources and respective thermal maturity. The crude oil samples are divided into two main families (A and B) based on differences in source inputs and thermal maturity. Using source specific parameters including pristane/phytane (Pr/Ph), hopane/sterane, dibenzothiophene/ phenanthrene (DBT/P), Pr/n-C17 and Ph/n-Cl8 ratios and the distributions of tricyclic and tetracyclic terpanes, family B oils are ascribed a marine source rock deposited under sub-oxic conditions, while family A oils have a more terrigenous source affinity. This genetic classification is supported by the stable carbon isotopic compositions (d13C) of the n-alkanes. Using biomarker maturity parameters such as the abundance of Pr and Ph relative to n-alkanes and the distribution of sterane and hopane isomers, family A oils are shown to be more thermally mature than family B oils. The contrasting maturity of the two families is supported by differences between the stable hydrogen isotopic compositions (dD) of Pr and Ph and the n-alkanes, as well as the d13C values of n-alkanes in their respective oils. 

Handover latency is the primary cause of packet loss resulting in performance degradation of standard Mobile IPv6. Mobile IPv6 with fast Handover enables a Mobile Node (MN) to quickly detect at the IP layer that it has moved to a new subnet by receiving link-related information from the link-layer; furthermore it gathers anticipative information about the new Access Point (AP) and the associated subnet prefix when the MN is still connected to the previous Corresponding Node (CN).

This paper proposes an enhancement to Fast Mobile IPv6 handover (FMIPv6), based on link layer information, we also present performance evaluations in terms of the packet loss and handover latency using evaluation models.

Abstract— Data security is an essential requirement, especially when sending information over a network. Network security has three goals called confidentiality, integrity and availability (or Access). Encryption is the most common technique used to achieve this goal. However, the computer society has not yet agreed on a standard method to measure data security. The ultimate goal of this study is to define security metrics based on different aspects of network security, and then demonstrate how these metrics could be used in Quality of Service (QoS) routing to find the most secure path connecting two distant nodes (source and destination) across an internetwork. Three security metrics are proposed in this document, these metrics have been derived from three important issues of network security, namely: authentication, encryption and traffic filtration techniques (firewalls and intrusion detection systems). The metrics follow different composition rules in that the first is binary, the second is either concave or additive and the last is multiplicative. Routing algorithms that make use of such metrics have been implemented in the C# programming language to test the viability of the proposed solution. Computational effort and blocking probability are the most commonly used performance measures were used to assess the behavior and the performance of these routing algorithms. Results obtained show that the algorithms were able to find feasible paths between communicating parties and helped in making reasonable savings in the computational effort needed to find an acceptable path. Consequently, higher blocking probabilities were encountered, which is thus the price to be paid for the savings.  

In this paper we want to show that each immediate basin of fixed points of Newton’s

function for a real polynomial is simply connected.

Keywords: Newton’s function, Immediate basin, Simply connected.

The title complex was isolated as red solid material from the reaction of Co(SCN)2 with 4(Salicylaldimine) antipyrine in ethanol. The molecular structure has been determined by elemental analysis, FT IR, UV-Vis and X-ray diffraction. The crystallographic data are: monoclinic

P21/n, a = 9.8335(3) A, b = 18.8593(6) A, c = 11.2921(4) A, = 90', =  = 90', V = 2041.46(12) ų and Z 2, R =0.045. The dicobalt (Il) complex is centrosymmetric dimer in which the Co(ll) ions are six coordinate being bonded to three coordinating donor sites from the corresponding tridentate Schiff base ligand and the fourth one is relatively bonded by phenolic oxygen, the fifth and sixth donor sites comes from isothiocyanate and methanol.