A large body of evidence now implicates increased leukocyte-endothelial cell adhesion as a key early event in the development of diabetic retinopathy. We recently reported that raised activity of the glycosylating enzyme core 2 beta 1,6-N-acetylglucosaminyltransferase (GlcNAc-T) through protein kinase C (PKC)beta2-dependent phosphorylation plays a fundamental role in increased leukocyte-endothelial cell adhesion and capillary occlusion in retinopathy. In the present study, we demonstrate that following exposure to plasma from diabetic patients, the human promonocytic cell line U937 exhibits a significant elevation in core 2 GlcNAc-T activity and increased adherence to cultured retinal capillary endothelial cells. These effects of diabetic plasma on enzyme activity and cell adhesion, mediated by PKCbeta2-dependent phosphorylation of the core 2 GlcNAc-T protein, were found to be triggered by increased plasma levels of tumor necrosis factor (TNF)-alpha. Levels of enzyme activity in plasma-treated U937 cells were closely dependent on the severity of diabetic retinopathy, with the highest values observed upon treatment with plasma of patients affected by proliferative retinopathy. Furthermore, we noted much higher correlation, as compared with control subjects, between increased values of core 2 GlcNAc-T activity and cell adhesion properties. Based on the prominent role of TNF-alpha in the development of diabetic retinopathy, these observations further validate the significance of core 2 GlcNAc-T in the pathogenesis of capillary occlusion, thereby enhancing the therapeutic potential of specific enzyme inhibitors.

 carbon materials2 through their unique combination of excellent processability and high carbon yield. The enediyne functionality of the monomers undergo a thermal Bergman cycloaromatization reaction that yields reactive naphthalene diradicals which polymerize to form polynapthalene.(Figure 1) The tetrafunctionality of the monomers allows for both a higher processing window due to extensive branching and ultimately the formation of network polymers. The high carbon yield results in less shrinkage of the polymer upon pyrolysis to the glassy carbon state. This allows for the faithful templating of carbon structures from a polymeric precursor.

Hydrogen fuel cell electrodes require several properties for optimum performance. An ideal electrode would have as high a surface area as possible with an uniform dispersion of nano-scale catalyst particles attached to the surface. The electrode must be electrically conductive and have good mass transport for products and reactants. Carbon supported platinum is the best known catalyst for the oxidation of hydrogen at the anode and the reduction of oxygen at the cathode of a proton exchange membrane fuel cell (PEMFC) 3. The material also must have good compatibility with the material used for the proton exchange membrane in the membrane electrode assembly (MEA), usually a sulfonated fluoropolymer such as Nafion. We have undertaken a study to prepare a high surface area carbon material through a BODA templating method which can then be functionalized with both well dispersed platinum nanoparticles …

Irregular (so-called broken) plural identification in modern standard Arabic is a problematic issue for information retrieval (IR) and language engineering applications, but their effect on the performance of IR has never been examined. Broken plurals (BPs) are formed by altering the singular (as in English: tooth→ teeth) through an application of interdigitating patterns on stems, and singular words cannot be recovered by standard affix stripping stemming techniques. We developed several methods for BP detection, and evaluated them using an unseen test set. We incorporated the BP detection component into a new light-stemming algorithm that conflates both regular and broken plurals with their singular forms. We also evaluated the new light-stemming algorithm within the context of information retrieval, comparing its performance with other stemming algorithms.

Abstract

Due to the high number of inflectional variations of Arabic words, empirical results suggest that stemming is essential for Arabic information retrieval. However, current light stemming algorithms do not extract the correct stem of irregular (so-called broken) plurals, which constitute ~10% of Arabic texts and ~41% of plurals. Although light stemming in particular has led to improvements in information retrieval [5, 6], the effects of broken plurals on the performance of information retrieval systems has not been examined.We propose a light stemmer that incorporates a broken plural recognition component, and evaluate it within the context of information retrieval. Our results show that identifying broken plurals and reducing them to their correct stems does result in a significant improvement in the performance of information retrieval systems.

после добавления токсиканта, во всем диапазоне концентраций фенола. Таким образом, зависимости, получаемые в таких случаях, являются сложными и фиксируют большое число факторов. Исключение в эксперименте одних факторов и изучение влияния других достигается в проточных реакторах полного перемешивания, когда один из факторов поддерживается постоянным (например, режим рН-стата). Построенные в работе зависимости позволяют разрабатывать методики эксперимента, служащих целям токсикологии, например, при разработке системы предельно допустимых концентраций по влиянию токсиканта на скорость дыхания тест-объекта. Природные фенольные соединения расщепляются почвенными микроорганизмами, использующими их в качестве источника углерода [29]. Микроорганизмы природных биоценозов приспособлены к низким концентрациям этих соединений. Для удаления фенолов при их высокой концентрации (например, промышленные сточные воды, аварийные ситуации, залповые выбросы) необходимо выделять формы, которые активно окисляют фенол, и адаптировать их к высоким концентрациям фенола как субстрата [30]. В связи с этим возникает круг задач оптимального сочетания концентраций основного субстрата, окислителя и косубстратов (азот в форме дрожжевого экстракта), которые могут быть решены в соответствии с подходом, представленным выше в форме задачи о конкуренции молекул субстрата и окислителя за активные центры поверхности клеточной мембраны. Круг подобного типа задач может быть расширен в зависимости от вопросов, которые ставит практика микробиологии.

Increased leukocyte-endothelial cell adhesion is a key early event in the development of retinopathy and atherogenesis in diabetic patients. We recently reported that raised activity of glycosylating enzyme [beta]1,6 acetylglucosaminyltransferase (core 2 GlcNAc-T) is responsible for increased leukocyte-endothelial cell adhesion and capillary occlusion in retinopathy. Here, we demonstrate that elevated glucose increases the activity of core 2 GlcNAc-T and adhesion of human leukocytes to retinal capillary endothelial cells, in a dose-dependent manner, through diabetes-activated serine/threonine protein kinase C beta2 (PKCbeta2)-dependent phosphorylation. This regulatory mechanism, involving phosphorylation of core 2 GlcNAc-T, is also present in polymorphonuclear leukocytes isolated from type 1 and type 2 diabetic patients. Inhibition of PKCbeta2 activation with the specific inhibitor, LY379196, attenuated serine phosphorylation of core 2 GlcNAc-T and prevented increased leukocyte-endothelial cell adhesion. Raised activity of core 2 GlcNAc-T was associated with a threefold increase in O-linked glycosylation of P-selectin glycoprotein ligand-1 on the surface of leukocytes of diabetic patients compared with age-matched control subjects. PKCbeta2-dependent phosphorylation of core 2 GlcNAc-T may thus represent a novel regulatory mechanism for activation of this key enzyme in mediating increased leukocyte-endothelial cell adhesion and capillary occlusion in diabetic retinopathy.

The preparation and characterization of a cobalt substituted ETS-10 titanosilicate are described. X-ray diffraction shows that cobalt incorporation causes an increase in unit cell dimensions. UV–VIS, EPR, Raman and Co K-edge XANES spectra all show that Co2+ occupies tetrahedral sites, substituting for silicon. The 29Si NMR spectra do not permit identification of which silicon sites in ETS-10 are substituted, but the Co K-edge EXAFS shows clearly that Co2+ substitutes at Si(3Si,1Ti) sites.

A review is given of recent work in the authors' laboratory on the characterization of different forms of nanostructured titania. It is shown that nanocrystalline anatase powders and nanocrystalline anatase thin films differ significantly in their optical properties, due primarily to differences in sintering behaviour on drying. It is argued that the electronic properties of these systems are determined by surface phenomena rather than quantum size effects. The novel titanosilicate zeolite ETS-10 which contains one dimensional “quantum wires” of titania provides an alternative system for studying quantum size effects which has considerable potential for photocatalysis.

The development of Language Engineering and Information Retrieval applications for Arabic require availability of sizeable, reliable corpora of modern Arabic text. These are not routinely available. This paper describes how we constructed an 18.5 million word corpus from Al-Hayat newspaper text, with articles tagged as belonging to one of 7 domains. We outline the profile of the data and how we assessed its representativeness. The literature suggests that the statistical profile of Arabic text is significantly different from that of English in ways that might affect the applicability of standard techniques. The corpus allowed us to verify a collection of experiments which had, so far, only been conducted on small, manually collected datasets. We draw some comparisons with English and conclude that there is evidence that Arabic data is much sparser than English for the same data size.

The exact mechanism for capillary occlusion in diabetic retinopathy is still unclear, but increased leukocyte-endothelial cell adhesion has been implicated. We examined the possibility that posttranslational modification of surface O-glycans by increased activity of core 2 transferase (UDP-Glc:Galbeta1-3GalNAcalphaRbeta-N-acetylglucoaminyltr ansferase) is responsible for increased adhesion of leukocytes to vascular endothelium in diabetes. The mean activity of core 2 transferase in polymorphonuclear leukocytes isolated from type 1 and type 2 diabetic patients was higher compared with age-matched control subjects (1,638 +/- 91 [n = 42] vs. 249 +/- 35 pmol x h(-1) x mg(-1) protein [n = 24], P = 0.00013; 1,459 +/- 194 [n = 58] vs. 334 +/- 86 [n = 11], P = 0.01). As a group, diabetic patients with retinopathy had significantly higher mean activity of core 2 transferase compared with individuals with no retinopathy. There was a significant association between enzyme activity and severity of retinopathy in type 1 and type 2 diabetic patients. There was a strong correlation between activity of core 2 transferase and extent of leukocyte adhesion to cultured retinal capillary endothelial cells for diabetic patients but not for age-matched control subjects. Results from transfection experiments using human myelocytic cell line (U937) demonstrated a direct relationship between increased activity of core 2 transferase and increased binding to cultured endothelial cells. There was no relationship between activity of core 2 transferase and HbA(1c) (P = 0.8314), serum advanced glycation end product levels (P = 0.4159), age of the patient (P = 0.7896), and duration of diabetes (P = 0.3307). On the basis that branched O-glycans formed by the action of core 2 transferase participate in leukocyte adhesion, the present data suggest the involvement of this enzyme in increased leukocyte-endothelial cell adhesion and the pathogenesis of capillary occlusion in diabetic retinopathy.